The potato mop-top virus TGB2 protein and viral RNA associate with chloroplasts and viral infection induces inclusions in the plastids

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The potato mop-top virus TGB2 protein and viral RNA associate with chloroplasts and viral infection induces inclusions in the plastids

The potato mop-top virus (PMTV) triple gene block 2 (TGB2) movement proteins fused to monomeric red fluorescent protein (mRFP-TGB2) was expressed under the control of the PMTV subgenomic promoter from a PMTV vector. The subcellular localizations and interactions of mRFP-TGB2 were investigated using confocal imaging [confocal laser-scanning microscope, (CLSM)] and biochemical analysis. The resul...

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Evolutionary features of 8K (KDa) silencing suppressor protein of Potato mop-top virus

The cysteine-rich 8K protein of Potato mop-top virus (PMTV) suppresses host RNA silencing. In this study, evolutionary analysisof 8K sequences of PMTV isolates was studied on the basis of nucleotide and amino acid sequences. Twenty-one positively selected sites were identified in 8K codingregions. Recombination events were found in the 8K of PMTV isolates with a rate of 1.8. Totally 30 haplotyp...

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ژورنال

عنوان ژورنال: Frontiers in Plant Science

سال: 2012

ISSN: 1664-462X

DOI: 10.3389/fpls.2012.00290